Mass multiplication of Bacillus subtilis
Preparation of mother culture
The nutrient broth medium is prepared as detailed below
Glucose : 5.0 g
Peptone : 5.0 g
Beef extract : 3.0 g
Sodium chloride : 3.0 g
Distilled water : 1000 ml
The above medium is dispensed in conical flasks and autoclaved at 15 lb pressure for 15 mts. A loop of B.subtilis is inoculated into the medium and incubated for 2 days. This serve as the mother culture.
Mass multiplication:
The nutrient broth is prepared in fermentor and sterilized at 15 lb pressure for 15 mts. Then the mother culture is added @ 1 lit / 100 lit of the medium and incubated at room temperature for 2 days. The medium containing the bacterial growth of B.subtilis is used for mixing with talc powder.
|